Advances in biotechnology necessitate both an understanding of scientific principles and ethical implications to be clinically applicable in medicine. In this regard, therapeutic cloning offers significant potential in regenerative medicine by circumventing immunorejection, and in the cure of genetic disorders when used in conjunction with gene therapy. Therapeutic cloning in the context of cell replacement therapy holds a huge potential for de novo organogenesis and the permanent treatment of Parkinson's disease, Duchenne muscular dystrophy, and diabetes mellitus as shown by in vivo studies. Scientific roadblocks impeding advancement in therapeutic cloning are tumorigenicity, epigenetic reprogramming, mitochondrial heteroplasmy, interspecies pathogen transfer, low oocyte availability. Therapeutic cloning is also often tied to ethical considerations concerning the source, destruction and moral status of IVF embryos based on the argument of potential. Legislative and funding issues are also addressed. Future considerations would include a distinction between therapeutic and reproductive cloning in legislative formulations.
Keywords: SCNT; biomedical ethics; cell replacement therapy; gene therapy; mitochondrial heteroplasmy; oocyte availability; therapeutic cloning.
Dhai A, Moodley J, McQuoid-Mason DJ, Rodeck C. Dhai A, et al. S Afr Med J. 2004 Nov;94(11):906-9. S Afr Med J. 2004. PMID: 15587453
Zhang ZP, Zhang JT, Huang SC, He XY, Deng LX. Zhang ZP, et al. Stem Cell Res Ther. 2020 Sep 7;11(1):388. doi: 10.1186/s13287-020-01907-0. Stem Cell Res Ther. 2020. PMID: 32894201 Free PMC article. Review.
French AJ, Wood SH, Trounson AO. French AJ, et al. Stem Cell Rev. 2006;2(4):265-76. doi: 10.1007/BF02698053. Stem Cell Rev. 2006. PMID: 17848713
Blesa JR, Tudela J, Aznar J. Blesa JR, et al. Linacre Q. 2016 May;83(2):179-191. doi: 10.1080/00243639.2016.1180773. Linacre Q. 2016. PMID: 27833197 Free PMC article.
de Miguel-Beriain I. de Miguel-Beriain I. Adv Drug Deliv Rev. 2015 Mar;82-83:176-80. doi: 10.1016/j.addr.2014.11.011. Epub 2014 Nov 14. Adv Drug Deliv Rev. 2015. PMID: 25446134 Review.
Arutyunyan IV, Fatkhudinov TK, Makarov AV, Elchaninov AV, Sukhikh GT. Arutyunyan IV, et al. World J Gastroenterol. 2020 Jun 14;26(22):2948-2966. doi: 10.3748/wjg.v26.i22.2948. World J Gastroenterol. 2020. PMID: 32587441 Free PMC article. Review.
Rao RV. Rao RV. J Ayurveda Integr Med. 2018 Jul-Sep;9(3):225-232. doi: 10.1016/j.jaim.2017.10.002. Epub 2017 Dec 21. J Ayurveda Integr Med. 2018. PMID: 29276113 Free PMC article. Review.
Nabavizadeh SL, Mehrabani D, Vahedi Z, Manafi F. Nabavizadeh SL, et al. World J Plast Surg. 2016 Sep;5(3):213-225. World J Plast Surg. 2016. PMID: 27853684 Free PMC article. Review.
Ayala FJ. Ayala FJ. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):8879-86. doi: 10.1073/pnas.1501798112. Epub 2015 Jul 20. Proc Natl Acad Sci U S A. 2015. PMID: 26195738 Free PMC article. Review.